| Autor: |
Yong Xie, Kai-Tai Tse, Zi Chen, Wai-Yee Sin, Yi Xie, Yan Yuan, Yan Ke, Bofu Xue |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Transfusion. 53:2001-2011 |
| ISSN: |
0041-1132 |
| Popis: |
Background Autologous and allogeneic hematopoietic stem cell (HSC) transplantations serve as effective therapy for a variety of hematologic and other diseases. Umbilical cord blood (UCB) is an important source of HSCs. However, it is difficult to obtain a sufficient number of HSCs with complete self-renewal capability derived from a single unit of UCB for use in adult transplantation. In this study, we investigated two novel three-dimensional (3D) culture systems (static and rotating) for ex vivo expansion of HSCs from UCB. Study Design and Methods We encapsulated the human cord blood mononuclear cells (CBMCs) in alginate 3D static and rotating culture systems, compared the cell number amplification, the proportion of CD34+ cells, and the colony-forming capacity of these systems to those of the conventional two-dimensional (2D) system. The amplified cells were transplanted into nonobese diabetic/severe combined immunodeficient (NOD/SCID) mice to confirm the hematopoiesis reconstruction capacity of the cells. Results The increase in the cell number and the proportion of CD34+ cells in the CBMCs was more effective in these 3D alginate culture systems than in the conventional 2D culture system under the same conditions (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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