Evaluation of our self-designed nanometer silicon membrane sandwich cup system for diagnosing tuberculosis
| Autor: | Zijuan Jian, Wan-chan Peng, Wenen Liu, Qingxia Liu, Xianghui Liang, Jun Peng, Hongling Li, Yanbing Li, Ke Hu |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pulmonary and Respiratory Medicine Pathology medicine.medical_specialty Tuberculosis business.industry 030106 microbiology Direct microscopy Membrane filter Gold standard (test) medicine.disease Silicon membrane 03 medical and health sciences medicine Clinical value Immunology and Allergy Sputum Direct smear medicine.symptom business Nuclear medicine Genetics (clinical) |
| Zdroj: | The Clinical Respiratory Journal. 10:647-652 |
| ISSN: | 1752-6981 |
| DOI: | 10.1111/crj.12273 |
| Popis: | Background and Aims A nanometer silicon membrane sandwich cup system was self-designed. It could concentrate the bacilli via 0.45-μm microporous filter membrane and semi-automate the acid-fast bacilli (AFB) by a bacteria-staining machine. The aim of the study was to assess the clinical value of our self-designed system for diagnosing tuberculosis (TB). Methods A total of 1993 sputum specimens obtained from patients with confirmed or suspected TB were subjected to direct or concentrated specimens smear at XiangYa Hospital, Central South University between May 2012 and February 2013. In addition, all the specimens were also inoculated into Lowenstein–Jensen (L-J) media, and culture results were considered as the gold standard for calculating sensitivity and specificity. Results Compared with direct smear examination, an increased density of red stained bacilli was observed in the self-designed nanometer silicon membrane sandwich cup analysis under the microscope. The positive rate of the self-designed analysis was significantly higher than that of direct AFB smear [10.9% (217/1993) vs 6.2% (123/1993), P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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