Multidrug Resistant Shigella Associated with Class 1 Integrase and Other Virulence Genes as a Cause of Diarrhea in Pediatric Patients
| Autor: | Samah Sabry El-Kazzaz, Ghada El-Saeed Mashaly, Mayada S. Zeid |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
biology medicine.drug_class 030106 microbiology Antibiotics Virulence medicine.disease_cause Microbiology Integrase Multiple drug resistance 03 medical and health sciences Diarrhea 0302 clinical medicine Multiplex polymerase chain reaction medicine biology.protein Shigella 030212 general & internal medicine medicine.symptom Gene |
| Zdroj: | Open Journal of Medical Microbiology. 10:1-16 |
| ISSN: | 2165-3380 2165-3372 |
| DOI: | 10.4236/ojmm.2020.101001 |
| Popis: | Background: Shigella is one of the most serious pathogens associated with bloody diarrhea in children. The empiric antibiotic therapy of enteric illness with blood streaked stool leads to emergence of multi drug resistant (MDR) Shigella. The condition gets exacerbated by presence of integrons that facilitate the horizontal spread. Virulence genes associated with MDR Shigella modulate the patient outcome, particularly in children. Objectives: The present study was aiming at isolation of MDR Shigella from children with diarrheal sickness and characterization of those isolates as regarding presence of class 1 integrase and other virulence genes. Methods: Four hundred and ninety patients under the age of five suffering from diarrheal illness were examined for presence of Shigella in their stool specimens. MDR Shigella was determined using the antibiotic susceptibility testing by disc diffusion method; those isolates were tested for presence of class 1 integrase by PCR. Multiplex PCR assay was used to determine the presence of virulence genes, virA, ial, sen, set1A, set1B, sat, ipaBCD, ipaH and stx in the MDR Shigella isolates. Results: The isolation rate of Shigella from pediatric patients was 5.3%. Most of the isolated Shigella (57.7%) were from infants between 12 and 23 month. 73.1% of the identified Shigella were MDR. intI1 gene was present in 78.9% of MDR isolates. Muliplex PCR revealed that ipaH and ipaBCD, virA, sat, ial, set1A and set1B, sen were detected in 94.7%, 78.9%, 73.7%, 68.4%, 42.1%, 36.8% of the MDR Shigella isolates respectively. Conclusion: The MDR isolates represented a considerable percentage of Shigella detected in pediatric patients. Presence of intI1 gene in most of MDR Shigella reflects the higher possibility of resistant strains spread. Existence of a variety of virulence genes in those isolates is an important indicator of serious disease outcome. |
| Databáze: | OpenAIRE |
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