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Introduction: In Nigeria, limited data exist to elucidate the inter-transmission of tuberculosis (TB) between humans and cattle. The study aimed at determining the circulating spoligotypes of Mycobacterium tuberculosis complex in cattle and humans, in Maiduguri.Methods: We conducted a cross sectional study on bovine and human tuberculosis in Maiduguri, Borno state. We calculated sample size using the method Thrusfield and Lesions suggestive of TB from 160 slaughtered cattle was obtained from Maiduguri Central Abattoir. Sputum samples from humans; 82 abattoir workers and 147 suspected TB patients from Hospitals/clinics were obtained. Lesions and sputum samples were cultured for the isolation of Mycobacterium spp. Positive cultures were subjected to deletion analysis and selected isolates were spoligotyped. Results: Fifty-two isolates were obtained from cattle. Of these, 26 (50%) belonged to M. tuberculosis complex (MTC) of which 17/26 (65.4 %) were characterized as M. bovis. In humans, seven of 12 (58.3%) MTC obtained were characterized as M. tuberculosis. Spoligotyping revealed SB0944 and SB1025 in cattle, while SIT838, SIT61 of LAM10_CAM and SIT1054, SIT46 of Haarlem (H) families were obtained from humans. Conclusions: Our findings reiterate the dominance of SB0944 and SB1025 in cattle in Nigeria. We isolated M. tuberculosis strain of the H family mainly domiciled in Europe from humans. |
