| Popis: |
Publisher Summary Among the coenzymes and regulatory compounds involved in biochemical reactions, adenine nucleotides are present in a large proportion of cases. Adenosine triphosphate and the corresponding diphosphate are required for many kinase reactions, in which a phosphoryl group is transferred to a variety of substrates; and adenosine is part of the coenzymes, DPN and TPN, that participate in most dehydrogenase reactions. In addition, these compounds are important in activating or inhibiting the activity of a number of regulatory enzymes. This chapter also explains preparation of adenosine analogs, and examples of reactions of 3'-FSBA and 5'-FSBA with particular proteins. It concludes that 3'- and 5'-FSBA may be useful in the affinity labeling of receptor proteins as well as of the regulatory and active sites of enzymes. Within the constraints imposed by the limitations of solubility and stability of these compounds, the fluorosulfonylbenzoyladenosines thus may have broad applicability in the labeling of adenine nucleotide sites in proteins. |
