Asociación entre los criterios de clasificación genotípica de Staphylococcus aureus resistente a meticilina que se obtuvo mediante electroforesis en geles de campos pulsantes y mediante reacción en cadena de la polimerasa de regiones hipervariables del gen mecA
| Autor: | Pamela Araya, Gustavo Medina, Juan Carlos Hormazabal, Heriberto Fernández, Jorge Fernández, M. Wilson, Laura Otth, Aurora Maldonado, Carola Otth |
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| Rok vydání: | 2009 |
| Předmět: |
Microbiology (medical)
Nosocomial outbreak Hospitalized patients SCCmec biochemical phenomena metabolism and nutrition Biology bacterial infections and mycoses medicine.disease_cause law.invention Microbiology law Staphylococcus aureus Genotype medicine Pulsed-field gel electrophoresis Degree of association Polymerase chain reaction |
| Zdroj: | Enfermedades Infecciosas y Microbiología Clínica. 27:213-218 |
| ISSN: | 0213-005X |
| DOI: | 10.1016/j.eimc.2008.08.001 |
| Popis: | Introduction: The emergence of methicillin-resistant Staphylococcus aureus (MRSA) has become a major epidemiological problem worldwide. Methods: We determined the degree of association between the genotype screening results obtained by pulse-field gel electrophoresis (PFGE) and polymerase chain reaction (PCR) for 7 hypervariable DNA regions associated with the mecA gene (HVR-mecA PCR), in the epidemiological analysis of 36 MRSA strains unrelated to nosocomial outbreaks, isolated from hospitalized patients at the County Hospital of Valdivia (Chile). Results: The strains were classified into 15 pulse types (A-O) and 5 genotypes (6, 14, 15, 16, and 17) by PFGE and HVR-mecA PCR, respectively. Most of the strains were grouped in pulse types D, E and I, which presented 85.7% similarity. The most common genotypes were 14 (36.1%) and 15 (33.3%). Each genotype |
| Databáze: | OpenAIRE |
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