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Salvia miltiorrhiza Bge.is a medicinal plant and perennial herb with relieving pain promoting blood circulation,and so on.In recent years,to supplying people′s the demand,the area of cultivation Salvia was increasing in Hebei province,but the quality was reducing because of non-standard planting and improper use of fertilizer and pesticides.Therefore,it has become the popular areas of researches to improve yield and quality of Salvia at home and abroad. Arbuscular mycorrhizal(AM) fungi are a class of beneficial soil microbes.It is well known that AM fungi can enhance the resistance of plants to stressful situations,such as drought,salt,nutrient deficiency,etc.At the same time,AM fungi can form a good symbiotic relationship with the almost ninety percent of vascular plants.Some researches have shown that the arbuscular mycorrhizal symbiosis has significant interactive effects with water and fertilizer.The paper mainly studied the effects of arbuscular mycorrhizal fungi(Glomus mosseae) on the growth and nutrient of Salvia miltiorrhiza under different soil water and fertilizer conditions with pot experiment in greenhouse.The experimental design include two water treatments(drought stress,40% of soil relative water content;normal water,70% of soil relative water content).The amount of phosphorus included three levels-low phosphorus(0.08 gP/kg soil,P1),normal phosphorus(0.16 gP/kg soil,P2) and high phosphorus(0.24 gP/kg soil,P3).The same condition inoculated differently with Glomus mosseae and non-mycorrhizal inoculation. The results showed that the treatment inoculated with AM fungi significantly promoted growth of host plant and infection rate,but the effect sof inoculation were affected by soil water and P level.Under 40% relative water content and different P levels,the inoculation of AM fungi on host plant has different influence on two water levels,Results as follows.The content of tanshinone in root significantly increased under P1,P2,P3 level;Both the content of tanshinone,Ca,Fe in shoot and Zn in root clearly went up only under P1,P2 level;The content of K,Zn in shoot increse only with P3 level;And the Mn in shoot increased only under P2,P3 level;However,when under P1,P2,P3 level,the content of danshensu in shoot and root,total phenolic and total flavonoids in root significantly reduced;Only with P1,P2 level,the total flavonoids in shoot decreased obviously..Under 70% relative water content,inoculation of AM fungi on Salvia miltiorrhiza Bge.has different influence on two water levels as follows.The content of total flavonoids,total phenolic in shoot and root,tanshinone,Fe in shoot and Ca,Mn in shoot all increased under P1,P2,P3 level;The content of danshensu and Zn in root also rised but only under P1,P2 level;The content of Mn in root increased only under P2,P3 level;Cu in shoot,Ca in root and K in shoot significantly increased under P1,P2 and P3 level respectively.The growth of Salvia miltiorrhiza Bge.was inhabited by water stress,but the inhibition was alleviated after inoculated with AM fungi.Therefore,AM fungi can form a good symbiotic relationship with Salvia miltiorrhiza Bge..Inoculation of AM fungi on the Salvia miltiorrhiza Bge.had the best effection under 70% relative water content and P2(0.16 gP/kg soil) level. |
