Genomes of Potato Mop-Top Virus (Virgaviridae: Pomovirus) Isolates from New Zealand and Their Impact on Diagnostic Methods

Autor:	Rebekah A. Frampton, Shea M. Addison, Falk Kalamorz, Grant R. Smith
Rok vydání:	2022
Předmět:	Plant Science Agronomy and Crop Science
Zdroj:	Plant Disease. 106:2571-2575
ISSN:	1943-7692 0191-2917
Popis:	Following the detection of potato mop-top virus (PMTV) in New Zealand in 2018, three near-complete PMTV genomes (AS22, AS99, AS144) were assembled from soil samples taken from potato fields in Canterbury. Phylogenetic analysis revealed that these genomes form a distinct lineage, with limited genetic diversity, within the PMTV species. This analysis supports the hypothesis that these genomes share a common origin, possibly resulting from a single (or limited) incursion of PMTV into New Zealand. A single nucleotide polymorphism was identified in the region where a key diagnostic primer binds. The mismatch of the diagnostic primer has implications for the effectiveness of the Mumford diagnostic protocol currently recommended for use in New Zealand; we recommend that the alternative Pandey assay, for which no primer mismatch was detected, be validated and optimized for use on the viral genomes present in New Zealand.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::33086327d6cd6a0481eb5a75540ada5c https://doi.org/10.1094/pdis-01-22-0192-sc Zobrazit plný text záznamu