Membrane permeability and intracellular disposition of51Cr(VI) in human red blood cells†

Autor:	H. Ottenwälder, H. M. Bolt, H.J. Wiegand
Rok vydání:	1987
Předmět:	Hemoglobin binding Chromatography Membrane permeability Chemistry Health Toxicology and Mutagenesis medicine.medical_treatment Kinetics chemistry.chemical_element Uptake kinetics Pollution Chromium medicine Environmental Chemistry Hemoglobin Saline Intracellular
Zdroj:	Toxicological & Environmental Chemistry. 14:219-226
ISSN:	1029-0486 0277-2248
DOI:	10.1080/02772248709357205
Popis:	Fast uptake kinetics of 51Cr(VI) into red blood cells (RBC) were studied. The uptake was determined as a process composed of two different first order processes with half lives of 23 sec and 10.4 min. Whereas the uptake of Cr(VI) in resuspended RBC (0.9% saline) was almost complete, in blood a portion of approximately 15% of the applied dose remained in plasma and did not penetrate into RBC, even after longer time periods. Over the concentration range studied (10 μM‐50mM), the fast initial uptake followed Michaelis‐Menten‐kinetics. The maximum capacity of this Cr(VI) transport into RBC of man was 3.1 × 108 ions × cell‐1 × min‐1. It is likely that Cr(VI) is transported under participation of “band‐3‐protein”;, a physiological anion carrier system of the RBC membrane. Intracellularly, chromium is almost quantitatively bound to hemoglobin, and thereby trapped for the cell's lifetime.
Databáze:	OpenAIRE
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