Three-Dimensional Epidermal Model from Human Hair Follicle-Derived Keratinocytes
| Autor: | Yuumi Nakamura, Michiyo Nakano, Ayako Oikawa, Takamitsu Matsuzawa, Hiroyuki Matsue |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
integumentary system Epidermis (botany) medicine.diagnostic_test Less invasive Biology Matrix (biology) Hair follicle Outer root sheath Cell biology 030207 dermatology & venereal diseases 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Biopsy medicine |
| Zdroj: | Skin Tissue Engineering ISBN: 9781493994724 |
| DOI: | 10.1007/978-1-4939-9473-1_10 |
| Popis: | Three-dimensional (3D) epidermal models reconstructed from human skin-derived keratinocytes have been utilized as an alternative to animal testing and models, not only in toxicology, but also in skin biology. Although there are currently several reconstructed human epidermis (RHE) models commercially available, the donors of the keratinocytes are not identified in these models. A tailor-made system is needed to investigate the individual differences in RHE derived from each donor.It is possible to make an individual RHE using each donor's keratinocytes, which are usually obtained by invasive procedures such as skin excision or biopsy. To overcome this drawback, we established an RHE model using keratinocytes derived from plucked hair follicles as a less invasive procedure under conditions without feeder cells, serum, or matrix proteins. In this chapter, we provide a method of isolation and two-dimensional (2D) culture of keratinocytes derived from adult human plucked hair follicles including the outer root sheath (ORS). We also provide a detailed protocol for establishing an RHE model by culturing the keratinocytes under a 3D culture condition. We believe that our less invasive technique will provide a useful tool for investigating individual RHE in both normal and disease settings. |
| Databáze: | OpenAIRE |
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