Freeze-Fracture Studies of Human Blood Platelets Activated by Thrombin Using Rapid Freezing

Autor:	J Leunissen-Bijvelt, Jan J. Sixma, Arie Verkley, Hans Hols
Rok vydání:	1985
Předmět:	integumentary system Chemistry Hematology Vacuole Freeze Fracturing Exocytosis law.invention Protoplasm Thrombin nervous system law medicine Biophysics Platelet Electron microscope Swelling medicine.symptom tissues medicine.drug
Zdroj:	Thrombosis and Haemostasis. 54:574-578
ISSN:	2567-689X 0340-6245
DOI:	10.1055/s-0038-1660073
Popis:	SummaryIn this study the influence of thrombin activation on human blood platelets has been followed by freeze-fracturing electron microscopy using rapid freezing in order to catch the initial changes in shape and the morphological alterations during the process of exocytosis of secretory granules. We found that isolation of the platelets by itself leads to some degree of shape change, which made it impossible to study the resting discoid platelet by rapid freezing.Activation of the platelets by thrombin induced dilation of the “surface connecting system (SCS)” with formation of large vacuoles as a result of fusion of the secretory granules with SCS. No intermediary fusion stages or structures were observed even using rapid freezing. Volcano-like protrusions and the corresponding complementary pits were seen at the SCS. These structures were interpreted by us as fractures through protoplasmic channels crossing the SCS. These channels originate during the swelling of the SCS as a result of the fusion of secretory granules with the SCS.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::32e6e4aefa4d4e9d8a838982155931a7 https://doi.org/10.1055/s-0038-1660073 Zobrazit plný text záznamu