An Improved Single-step Screening Method for Glucose-6-Phosphate Dehydrogenase Deficiency

Autor:	Hisaichi Fujii, Akira Hirono, Shiro Miwa
Rok vydání:	1998
Předmět:	Primaquine Chromatography Pipette Dehydrogenase Single step medicine.disease chemistry.chemical_compound chemistry Biochemistry medicine Screening method Formazan Nicotinamide adenine dinucleotide phosphate medicine.drug Glucose-6-phosphate dehydrogenase deficiency
Zdroj:	Japanese Journal of Tropical Medicine and Hygiene. 26:1-4
ISSN:	2186-1811 0304-2146
DOI:	10.2149/tmh1973.26.1
Popis:	We have established a new simple and rapid screening method for glucose-6-phosphate dehydrogenase (G6PD) deficiency. The principle of this method is the formation of blue formazan with the reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) produced by G6PD absorbed on a DEAE-Sephadex anion exchanger. The whole procedure is performed in a microcentrifuge tube and it can be completed in less than 30 min without any special equipment other than micropipettes. Our method is particularly suitable for field detection of G6PD-deficient subjects prior to administration of primaquine in situ.
Databáze:	OpenAIRE
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