Orientation Control of Photo-Immobilized Antibodies on the Surface of Azobenzene-Containing Polymers by the Introduction of Functional Groups
| Autor: | Taiji Ikawa, Mamiko Narita, Fumihiko Hoshino, Makoto Mouri, Osamu Watanabe |
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| Rok vydání: | 2010 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics Immobilized Antibodies Bioengineering Polymer Electrostatics Biomaterials Orientation control chemistry.chemical_compound Adsorption Azobenzene chemistry Polymer chemistry Materials Chemistry Surface charge Biotechnology Electrostatic interaction |
| Zdroj: | Macromolecular Bioscience. 10:612-620 |
| ISSN: | 1616-5187 |
| DOI: | 10.1002/mabi.200900394 |
| Popis: | In our photo-induced immobilization technique for an antibody (IgG) using azopolymers, the introduction of COOH and NMe(2) into the azopolymers, which can introduce surface charges, strongly affected the immobilization properties such as the efficiency of immobilization and the activity of the immobilized IgG (i.e., the orientation of the immobilized IgG). The introduction of COOH promoted a more active orientation of the immobilized IgG. The orientation was determined during the adsorption process onto the azopolymer surface in solution before photo-immobilization, and was maintained during the photo-immobilization. The surface charge of the azopolymer appears to be an important factor for IgG orientation, which involves electrostatic interactions between its Fab and the azopolymer surface. |
| Databáze: | OpenAIRE |
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