Histofluorometric assessment of insulin content in the endocrine tissue of the rat pancreas in the postreanimation period

Autor:	A. B. Aubakirov, A. A. Kikimbayeva, A. P. Andreyeva, A. N. Bazhanov, A. S. Lebedev
Rok vydání:	1996
Předmět:	medicine.medical_specialty Endocrinology business.industry Endocrinology Diabetes and Metabolism Period (gene) Internal medicine Insulin medicine.medical_treatment medicine Rat Pancreas Endocrine tissue business
Zdroj:	Problems of Endocrinology. 42:35-37
ISSN:	2308-1430 0375-9660
DOI:	10.14341/probl12044
Popis:	Histofluorometric measurement of insulin was carried out in В cells of the rat pancreas after pseudoisocyanine staining of slices in various terms of the postreanimation period following 10-min clinical death after V.G. Korpachev. The studies showed that the reanimation factor enhanced insulin release into the blood and changed the morphology of islet endocrine tissue of the gland. Hypoxia promoted transformation of mixed acinar insular, acinar glandular, and ductal epithelial cells into insulin-producing cells in experimental animals. Histofluorometric analysis of assessing insulin content in histological preparations gives reliable data on the production of this hormone in the glandular B-cells.
Databáze:	OpenAIRE
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