| Autor: |
Eike Reich, Albert T. Sneden |
| Rok vydání: |
1997 |
| Předmět: |
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| Zdroj: |
Journal of Chromatography A. 763:213-219 |
| ISSN: |
0021-9673 |
| DOI: |
10.1016/s0021-9673(96)00849-7 |
| Popis: |
Maytansinoids obtained from Maytenus species are known as potential anti-cancer drugs. Investigation of new plant species for the presence of unknown maytansinoids as well as synthetic work requires a reliable analytical and semipreparative normal-phase separation which can distinguish between known and unknown compounds independently from retention times and which would be compatible to LC-MS interfacing for further studies. A thorough study of numerous maytansinoids on silica gel and on diol-, aminopropyl- and cyanopropyl-bonded phases, suggests a complex retention mechanism based on molecular size as well as on specific interaction with the stationary phase while limiting a selectivity effect of the mobile phase. Utilizing a Varian 9065 photodiode array detector a spectral library of the separated substances was generated. This library simplifies the identification of known compounds in a matrix of unknowns such as fractions of plant extracts even if baseline separation is not achieved. Unlike reversed-phase separations that have been described in the literature the presented method allows an easy sample recovery in a semipreparative separation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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