Identification of a G1-S-phase-regulated region in the human thymidine kinase gene promoter

Autor:	Holger H. Roehl, Susan E. Conrad
Rok vydání:	1990
Předmět:	Regulation of gene expression Mutant Cell Biology Transfection Biology Molecular biology Primer extension chemistry.chemical_compound chemistry Transcription (biology) Thymidine kinase Thymidine Molecular Biology Gene
Zdroj:	Molecular and Cellular Biology. 10:3834-3837
ISSN:	1098-5549 0270-7306
DOI:	10.1128/mcb.10.7.3834
Popis:	The authors have identified a regulatory region in the human thymidine kinase gene promoter. A set of promoter deletion mutants was constructed, linked to the bacterial neomycin resistance gene, and stably transfected into Rat3 cells. It was shown that the region between 135 and 67 base pairs upstream of the cap site is required for conveying G{sub 1}-S-phase regulation to the linked {ital neo} gene. In addition, primer extension assays demonstrated that the same transcriptional start sites were used in G{sub 1}- and S-phase cells and in the various deletion mutants tested.
Databáze:	OpenAIRE
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