Production, characterization and dye decolorization ability of a high level laccase from Marasmiellus palmivorus
| Autor: | Roselei Claudete Fontana, Camila Cantele, Aline Ganzer Mezzomo, Marli Camassola, Aldo José Pinheiro Dillon, Liliane Poleto, Letícia Osório da Rosa |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Reaction conditions chemistry.chemical_classification Laccase Chromatography Laccase activity Marasmiellus palmivorus Continuous stirred-tank reactor Bioengineering 01 natural sciences Applied Microbiology and Biotechnology 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Enzyme chemistry 010608 biotechnology Phenol Thermal stability Agronomy and Crop Science Food Science Biotechnology |
| Zdroj: | Biocatalysis and Agricultural Biotechnology. 12:15-22 |
| ISSN: | 1878-8181 |
| DOI: | 10.1016/j.bcab.2017.08.012 |
| Popis: | Production of phenol oxidases by Marasmiellus palmivorus was evaluated in submerged cultures at different pH conditions: uncontrolled (free), fixed at 5.0, 6.0 and 7.0 and controlled after reaching pH 5.0, 6.0 and 7.0. pH (3–8) and temperature (20–45 °C) were optimized for laccase production and the thermal stability (30–60 °C) of laccases was evaluated. In addition, laccase activity in concentrated crude enzyme extract was evaluated. Laccase activity was greatest in production conditions of free pH (1950.6 ± 42.7 U mL−1), free - pH 6 (1901 ± 85.5 U mL−1) and fixed - pH 7 (1851.8 ± 0.0 U mL−1). Laccase activity was highest when reaction conditions were pH 4.0–5.5 and 25–45 °C. By concentrating the crude enzyme extract it was possible to significantly increase laccase activity. The greater the enzymatic activity used in the decolorization of the dyes Reactive Blue 220 and Acid Green 28, the shorter the time required to remove 90% and 75% of the color respectively. Given the results, it is evident that M. palmivorus can secrete high concentrations of phenol oxidases with potential biotechnological applications. |
| Databáze: | OpenAIRE |
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