| Autor: |
Al Husseini, Adel F. Al-Kholy, Naglaa Al-Toukhy, Omnia Al-Said, M. Magdi Atta, Amal Abou El-Fadle, Naglaa Fathy |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
American Journal of Biochemistry and Biotechnology. 7:55-62 |
| ISSN: |
1553-3468 |
| DOI: |
10.3844/ajbbsp.2011.55.62 |
| Popis: |
Problem statement: Hepatocellular carcinoma will emerge as a major fo rm of malignancy in the coming decades. The continuing high incidenc e of hepatocellular carcinoma, suggests that this disease will continue to represent a global health problem far into the future. Different genes encode for the various components of the human telomerase complex. These components include the human Telomerase RNA Component (hTERC) and the Telomerase Catalytic Subunit (hTERT). Correlation between Telomerase Reverse Transcriptase (hTERT) expression and telomerase activity has been reported in cancer patients. This work aimed to cla rify the significance of human Telomerase Reverse Transcriptase (hTERT mRNA) as a potential molecular tumor marker for Hepatocellular Carcinoma (HCC). Approach: The current study included 25 patients of hepatoce llular carcinoma (HCC), 30 patients with liver cirrhosis and 25 age and sex matched individuals with normal laboratory and Image findings as a control group. h TERT mRNA was measured in plasma by Real time PCR in all patients samples in comparison with normal healthy controls. Results: The expression of hTERT mRNA by relative unit was 129.10 ±27.6 with range (67.72-69.6) Vs 5245.87 ±2382.48 (2053-12232.90) Vs 92782.76 ±16158 (61783.25-118596.47) for control Vs cirrhosis Vs HCC group respectively. The hTERT expression was significantly with 699 and 33 fold increase in HCC and cirrhosis groups correspondingl y when compared to that of controls p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
