Bridge DNA amplification of cancer-associated genes on cross-linked agarose microbeads

Autor:	T. V. Nasedkina, D. O. Fesenko, Edward N. Timofeev, Natalia A. Kolganova, Alexander V. Chudinov, V. E. Kuznetsova, V. A. Vasiliskov, Anton E. Egorov, Alexander S. Zasedatelev
Rok vydání:	2014
Předmět:	musculoskeletal diseases Substrate (chemistry) Flow cell Dna amplification Molecular biology Analytical Chemistry stomatognathic diseases chemistry.chemical_compound chemistry Agarose Primer (molecular biology) Linker Gene DNA
Zdroj:	Microchimica Acta. 182:557-563
ISSN:	1436-5073 0026-3672
Popis:	A cross-linked agarose substrate was studied as a 3D support for bridge solid-phase DNA amplification (SPA). In this kind of SPA, primers are immobilized on agarose beads. Flow cell studies of SPA in real-time experiments showed that the amplification efficiency is strongly affected by (a) the presence of a linker between the primer and substrate, and (b) by the loading with primers. In fact, a high loading density may compromise SPA. The analysis of real time SPA curves using geometric growth model highlighted the advantage of 3D agarose support over the flat surfaces. The potential of bridge 3D SPA in DNA diagnostics was successfully demonstrated by on-chip analysis of mutations of the cancer-associated genes BRCA1/2 and CHEK2.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::328d8d6e1a102ade22277a85e7471a78 https://doi.org/10.1007/s00604-014-1357-8 Zobrazit plný text záznamu Full text from SpringerLink