The Characterization of Hemoglobin Manitoba or α2102(G9)Ser→Argβ2and Hemoglobin Contaldo or α2103(G10)HIS→ARGβ2By High Performance Liquid Chromatography
| Autor: | G. Ivaldi, G. V. Sciarratta, G. Sansone, A. L. Reese, M. L. Salkie, J. B. Wilson, Titus H.J. Huisman, G. L. Molaro |
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| Rok vydání: | 1984 |
| Předmět: |
Hemoglobin Contaldo
Performic acid Stereochemistry Hydrogen bond Biochemistry (medical) Clinical Biochemistry Hemoglobin Manitoba Hematology medicine.disease High-performance liquid chromatography chemistry.chemical_compound Hemoglobinopathy chemistry Biochemistry medicine Hb Manitoba Beta (finance) Genetics (clinical) |
| Zdroj: | Hemoglobin. 8:169-181 |
| ISSN: | 1532-432X 0363-0269 |
| DOI: | 10.3109/03630268408991710 |
| Popis: | Hb Contaldo with a HIS→ARG substitution at position 103(G10) of the α chain is a newly discovered unstable Hb variant observed in an Italian child. Its instability is probably due to the disruption of the hydrogen bond between α103(G10)His and β108(G10)Asn. The structural variation in the core segment was determined through analysis of tryptic peptides from digests of the αx and oxidized αx (with performic acid) chains, which were separated by HPLC. Similar analyses were made for the αX chain of the rare Hb Manitoba in which α102(G9) Ser is replaced by Arg. This variant was observed for the first time in an Italian patient, and was also studied in a member of a previously described Canadian family. |
| Databáze: | OpenAIRE |
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