Ultrasound-assisted hydrolysis of sugarcane bagasse using cellulolytic enzymes by direct and indirect sonication
| Autor: | Edson Luiz Foletto, Marcio A. Mazutti, Juliana Machado Gasparotto, Marco Antônio Mainardi, Sérgio Luiz Jahn, Raquel C. Kuhn, Leandro B. Werle |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
chemistry.chemical_classification
Chromatography Chemistry business.industry Sonication Ultrasound Bioengineering Applied Microbiology and Biotechnology Hydrolysis Enzyme Biochemistry Enzymatic hydrolysis Yield (chemistry) Fermentation Bagasse business Agronomy and Crop Science Food Science Biotechnology |
| Zdroj: | Biocatalysis and Agricultural Biotechnology. 4:480-485 |
| ISSN: | 1878-8181 |
| DOI: | 10.1016/j.bcab.2015.07.006 |
| Popis: | In this work was studied the enzymatic hydrolysis of non-treated sugarcane bagasse under direct and indirect sonication using cellulolytic enzymes produced by solid-state fermentation. Hydrolyzes were carried out using a high-intensity ultrasound probe system of 300 W cm−2 and 24 kHz. The effects of operational variables such as ultrasound intensity, pulse factor and enzyme concentration on the hydrolysis yield were investigated. Maximum yield of hydrolysis were 31.3 g kg−1 and 60.6 g kg−1 under direct and indirect sonication, respectively. The optimized condition for indirect sonication hydrolysis was enzyme concentration higher than 209.5 µl g−1, continuous ultrasound irradiation and ultrasound intensity of 60 W cm−2. The indirect ultrasound irradiation showed to be more promising technology to be used in enzymatic reaction due to its positive effects on hydrolysis yield. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect