Chemical Composition and in vitro Cytotoxic and Antileishmanial Activities of Extract and Essential Oil from Leaves of Piper cernuum
| Autor: | Patricia Sartorelli, Luiz Felipe Domingues Passero, Camyla F. Farias, Alisson L. Matsuo, Tabata M. Capello, Diogo Oliveira-Silva, Carlos R. Figueiredo, João Henrique G. Lago, Euder Glendes Andrade Martins |
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| Rok vydání: | 2015 |
| Předmět: |
Pharmacology
biology Traditional medicine Phenylpropanoid Chemistry Antiparasitic medicine.drug_class Plant Science General Medicine Fractionation Piper cernuum Piperaceae biology.organism_classification In vitro law.invention Complementary and alternative medicine law Drug Discovery medicine Chemical composition Essential oil |
| Zdroj: | Natural Product Communications. 10:1934578X1501000 |
| ISSN: | 1555-9475 1934-578X |
| DOI: | 10.1177/1934578x1501000217 |
| Popis: | Fractionation of the MeOH extract from leaves of Piper cernuum Vell. (Piperaceae) afforded six phenylpropanoid derivatives: 3′,4′-dimethoxy-dihydrocinnamic acid (1), piplaroxide (2), methyl 4′-hydroxy-3′,5′-dimethoxy cinnamate (3), 3′,4′,5′-trimethoxydihydrocinnamic acid (3), dihydropiplartine (5), and piplartine (6). The structures of isolated metabolites were characterized by NMR and MS spectral data analysis. The chemical composition of essential oil from the leaves was determined using GC/LREIMS followed by the determination of Kovats indexes. This procedure allowed the identification of nineteen terpenoids, with β-elemene (7), bicyclogermacrene (8), germacrene D (9), and (E)-caryophyllene (10) as the main compounds. Compounds 1 and 3-6 displayed no in vitro cytotoxicity against cancer cell lineages B16F10-Nex2, U87, HeLa, HL-60, HCT, and A2058 while 2 showed moderate activity against B16F10-Nex2 and HL-60 lines. Otherwise, compounds 7-10 displayed high cytotoxic activity. Evaluation against non-tumorigenic HFF cells indicated a reduced selectivity of compounds 7-10 to tumoral cells. No antileishmanial activity on macrophages infected with L. (L.) amazonensis was found for the crude MeOH extract and compounds 1-6. The crude essential oil and compounds 7-10 reduced parasitism and eliminated the majority of infected and non-infected cells at 50μg/mL. |
| Databáze: | OpenAIRE |
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