First Report of Corynespora cassiicola Causing Fruit Rot of Pomegranate in India, Its Morphological and Molecular Characterization
| Autor: | Milind Gajbhiye, Balu P. Kapadnis |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
biology Inoculation Biological pest control 02 engineering and technology Cellulase biology.organism_classification 01 natural sciences Enzyme assay Horticulture 0202 electrical engineering electronic engineering information engineering Xylanase biology.protein 020201 artificial intelligence & image processing Pectinase Corynespora cassiicola Engineering (miscellaneous) Pathogen 010606 plant biology & botany |
| Zdroj: | National Academy Science Letters. 42:253-257 |
| ISSN: | 2250-1754 0250-541X |
| DOI: | 10.1007/s40009-018-0722-2 |
| Popis: | A new fungal pathogen, Corynespora cassiicola was isolated from infected pomegranates from orchards of Maharashtra. It causes a mild form of rot in pomegranates. The pathogen was isolated from only 5.12% of infected pomegranates of Bhagwa and Ganesh varieties. It produced approximately 12 mm rot on artificially infected pomegranates and necrosis was 8 mm deep after 10 days post inoculation. The pathogen was identified by cultural and morphological characteristics that include growth on different media and slide culture technique. Further authentication was done by the sequencing of ITS1, 5.8S and ITS2 region of rRNA gene. The accession number of sequence submitted to NCBI is KC411916. The pathogen produced plant cell wall degrading enzymes viz., pectinase, cellulase and xylanase. In artificially infected tissues, pectinase activity was maximum (19.8 U/g) followed by xylanase (15.58 U/g) and cellulase (1.09 U/g). Pearson correlation indicated a significant correlation (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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