Biogenesis of silver nanoparticles using endophytic fungus Pestalotiopsis microspora and evaluation of their antioxidant and anticancer activities
| Autor: | Vijaya Tartte, M.S. Bethu, Vasudeva Reddy Netala, Bobbu Pushpalatha, Sani Aishwarya, Vijaya Bhaskar Baki, J. Venkateswara Rao |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Biophysics Pharmaceutical Science Bioengineering 02 engineering and technology Silver nanoparticle Microbiology Biomaterials 03 medical and health sciences chemistry.chemical_compound Biosynthesis Drug Discovery Microspora medicine Zeta potential biology Chinese hamster ovary cell Organic Chemistry Pestalotiopsis microspora General Medicine 021001 nanoscience & nanotechnology biology.organism_classification 030104 developmental biology chemistry Gymnema sylvestre 0210 nano-technology Nuclear chemistry |
| Zdroj: | International Journal of Nanomedicine. 11:5683-5696 |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s112857 |
| Popis: | An endophytic fungal strain isolated from the leaves of Gymnema sylvestre was identified as Pestalotiopsis microspora VJ1/VS1 based on nucleotide sequencing of internal transcribed spacer region (ITS 1-5.8S-ITS 2) of 18S rRNA gene (NCBI accession number {"type":"entrez-nucleotide","attrs":{"text":"KX213894","term_id":"1027316127","term_text":"KX213894"}}KX213894). In this study, an efficient and ecofriendly approach has been reported for the synthesis of silver nanoparticles (AgNPs) using aqueous culture filtrate of P. microspora. Ultraviolet-visible analysis confirmed the synthesis of AgNPs by showing characteristic absorption peak at 435 nm. Fourier transform infrared spectroscopy analysis revealed the presence of phenolic compounds and proteins in the fungal filtrate, which are plausibly involved in the biosynthesis and capping of AgNPs. Transmission electron microscopy (TEM) showed that the AgNPs were spherical in shape of 2–10 nm in size. Selected area electron diffraction and X-ray diffraction studies determined the crystalline nature of AgNPs with face-centered cubic (FCC) lattice phase. Dynamic light scattering analysis showed that the biosynthesized AgNPs possess high negative zeta potential value of −35.7 mV. Biosynthesized AgNPs were proved to be potential antioxidants by showing effective radical scavenging activity against 2,2′-diphenyl-1-picrylhydrazyl and H2O2 radicals with IC50 values of 76.95±2.96 and 94.95±2.18 µg/mL, respectively. The biosynthesized AgNPs exhibited significant cytotoxic effects against B16F10 (mouse melanoma, IC50 =26.43±3.41 µg/mL), SKOV3 (human ovarian carcinoma, IC50 =16.24±2.48 µg/mL), A549 (human lung adenocarcinoma, IC50 =39.83±3.74 µg/mL), and PC3 (human prostate carcinoma, IC50 =27.71±2.89 µg/mL) cells. The biosynthesized AgNPs were found to be biocompatible toward normal cells (Chinese hamster ovary cell line, IC50 =438.53±4.2 µg/mL). Cytological observations on most susceptible SKOV3 cells revealed concentration-dependent apoptotic changes that include cell membrane blebbing, cell shrinkage, pyknotic nuclei, karyorrhexis followed by destructive fragmentation of nuclei. The results together in this study strongly provided a base for the development of potential and versatile biomedical applications of biosynthesized AgNPs in the near future. |
| Databáze: | OpenAIRE |
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