GPRC5b Modulates Inflammatory Response in Glomerular Diseases via NF-κB Pathway
| Autor: | Jaakko Patrakka, Mark Lal, Annika Östman Wernerson, Xidan Li, Jenny Nyström, Katja Möller-Hackbarth, Sonia Zambrano, Patricia Q. Rodriguez, Angelina Schwarz, Emmanuelle Charrin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
biology Inflammation General Medicine 030105 genetics & heredity Podocyte Nephrin 03 medical and health sciences 030104 developmental biology medicine.anatomical_structure Downregulation and upregulation Nephrology Knockout mouse Cancer research biology.protein Glomerular Filtration Barrier medicine Signal transduction medicine.symptom Gene knockout |
| Zdroj: | Journal of the American Society of Nephrology. 30:1573-1586 |
| ISSN: | 1533-3450 1046-6673 |
| Popis: | Background Inflammatory processes play an important role in the pathogenesis of glomerulopathies. Finding novel ways to suppress glomerular inflammation may offer a new way to stop disease progression. However, the molecular mechanisms that initiate and drive inflammation in the glomerulus are still poorly understood. Methods We performed large-scale gene expression profiling of glomerulus-associated G protein-coupled receptors (GPCRs) to identify new potential therapeutic targets for glomerulopathies. The expression of Gprc5b in disease was analyzed using quantitative PCR and immunofluorescence, and by analyzing published microarray data sets. In vivo studies were carried out in a podocyte-specific Gprc5b knockout mouse line. Mechanistic studies were performed in cultured human podocytes. Results We identified an orphan GPCR, Gprc5b, as a novel gene highly enriched in podocytes that was significantly upregulated in common human glomerulopathies, including diabetic nephropathy, IgA nephropathy, and lupus nephritis. Similar upregulation of Gprc5b was detected in LPS-induced nephropathy in mice. Studies in podocyte-specific Gprc5b knockout mice showed that Gprc5b was not essential for normal development of the glomerular filtration barrier. However, knockout mice were partially protected from LPS-induced proteinuria and recruitment of inflammatory cells. Mechanistically, RNA sequencing in Gprc5b knockouts mice and experiments in cultured human podocytes showed that Gpr5cb regulated inflammatory response in podocytes via NF-kappa B signaling. Conclusions GPRC5b is a novel podocyte-specific receptor that regulates inflammatory response in the glomerulus by modulating the NF-kappa B signaling pathway. Upregulation of Gprc5b in human glomerulopathies suggests that it may play a role in their pathogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|