Aflatoxin detoxification in tortillas using an infrared radiation thermo-alkaline process: Cytotoxic and genotoxic evaluation
| Autor: | Abraham Méndez-Albores, Alicia Marroquin-Cardona, Juan de Dios Figueroa-Cárdenas, Patricia Ramírez-Noguera, Gerónimo Arámbula-Villa, Ana María Salazar, Anai Zavala-Franco, Monserrat Sordo |
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| Rok vydání: | 2020 |
| Předmět: |
Aflatoxin
010401 analytical chemistry food and beverages 04 agricultural and veterinary sciences medicine.disease_cause Malondialdehyde 040401 food science 01 natural sciences 0104 chemical sciences Ames test Lipid peroxidation Comet assay chemistry.chemical_compound 0404 agricultural biotechnology chemistry Nixtamalization medicine Food science Genotoxicity Carcinogen Food Science Biotechnology |
| Zdroj: | Food Control. 112:107084 |
| ISSN: | 0956-7135 |
| DOI: | 10.1016/j.foodcont.2019.107084 |
| Popis: | Aflatoxins are secondary metabolites with mutagenic, carcinogenic, teratogenic and immunosuppressive capacity in humans, the occurrence of which in maize grain is widespread. Nixtamalization, a process based on alkaline cooking, including infrared radiation, may be a suitable method for detoxification of this toxin. In this work, we carried out a cytotoxic and genotoxic evaluation of the extracts from maize (ME), tortilla from an infrared nixtamalization process (TEIR) and tortilla from a traditional nixtamalization process (TET) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, lipid peroxidation (malondialdehyde production), oxidative damage (glutathione modulation), Ames test (mutagenic response), and Comet assay (DNA damage). The formation capacity of the AFB1-Lysine (AFB1-Lys) adduct using maize and tortilla extracts was also tested. The infrared nixtamalization process showed a reduction of up to 93% of aflatoxins in tortillas, with a decrease in cytotoxicity and genotoxicity in all the tests performed. However, with TEIR and TET, AFB1-Lys adduct was not formed. We concluded that the process of nixtamalization with infrared radiation can be used for the detoxification of aflatoxins in tortillas. |
| Databáze: | OpenAIRE |
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