| Autor: |
Ines Bouhlel, L. Chekir Ghedira, Jihed Boubaker, Wissem Bhouri, Soumaya Kilani, Kamel Ghedira |
| Rok vydání: |
2011 |
| Předmět: |
|
| Zdroj: |
Cell Proliferation. 44:283-290 |
| ISSN: |
0960-7722 |
| DOI: |
10.1111/j.1365-2184.2011.00749.x |
| Popis: |
Objective: Kaempferol 3-O-β-isorhamninoside (K3O-ir) and rhamnocitrin 3-O-β-isorhamninoside (R3O-ir) from Rhamnus alaternus L leaves are investigated for their ability to induce apoptosis in human lymphoblastoid cells. We have attempted to characterize apoptotic pathway activated by these two flavonoids. Material and methods: Apoptosis of the human TK6 lymphoblastoid cell line was detected by DNA fragmentation, PARP cleavage and by evaluating caspase activity. Results: Apoptosis was observed after 24- and 48-h incubation of the cells with the tested compounds. DNA fragmentation was observed after treatment with flavonoids; this was confirmed by demonstration of PARP cleavage. Caspase-3 and caspase-8 activities were induced by both K3O-ir and R3O-ir flavonoids showing highest activity with compound concentration of 400 μg/ml. Conclusion: We have demonstrated that K3O-ir and R3O-ir induce apoptosis in human lymphoblastoid cells by the extrinsic pathway of apoptosis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
