| Autor: | Cs. Vágvölgyi, Ferenc Kevei, M. Rozs, László Manczinger |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Proteases
animal structures Protease biology Strain (chemistry) Physiology Sequence analysis Chromogenic medicine.medical_treatment General Medicine Isolation (microbiology) biology.organism_classification Applied Microbiology and Biotechnology Biochemistry medicine Bacillus licheniformis Bacteria Biotechnology |
| Zdroj: | World Journal of Microbiology and Biotechnology. 19:35-39 |
| ISSN: | 0959-3993 |
| DOI: | 10.1023/a:1022576826372 |
| Popis: | A keratin-degrading bacterium strain (K-508) was isolated from partially degraded feathers and characterized. This isolate exhibited a high chicken feather-degrading activity when cultured in feather-containing broth with a growth optimum of pH 7.0 and 47 °C. On the basis of its phenotypic characteristics (quickly moving, Gram-positive rods), the results of metabolic tests and rDNA sequence analysis, it was identified as Bacillus licheniformis. Its fermentation broth showed activity on N-Bz-l-Phe-l-Val-l-Arg-p-nitroanilide, N-Suc-l-Ala-l-Ala-l-Pro-l-Phe-p-nitroanilide, N-CBZ-Gly-Gly-l-Leu-p-nitroanilide and N-CBZ-l-Ala-l-Ala-l-Leu-p-nitroanilide as chromogenic protease substrates at near neutral pH. Both trypsin-like and chymotrypsin-like proteases were constitutively secreted by this strain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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