Evaluation of Ah Genotype on PAH: DNA Binding and Metabolite Excretion in Mice Fed Coal Tar

Autor:	Kimberly A. Nemeth, Eric H. Weyand
Rok vydání:	2002
Předmět:	medicine.medical_specialty Polymers and Plastics Chemistry Metabolite Organic Chemistry Urine medicine.disease_cause Adduct Excretion chemistry.chemical_compound Endocrinology Biochemistry Internal medicine DNA adduct Materials Chemistry medicine Pyrene Coal tar Genotoxicity medicine.drug
Zdroj:	Polycyclic Aromatic Compounds. 22:899-910
ISSN:	1563-5333 1040-6638
DOI:	10.1080/10406630290104068
Popis:	Female C57BL/6J and DBA/2J mice were fed a diet containing 0.3% coal tar for 14 days. Urine was collected during the last 24 hr of diet administration for metabolite analysis. Lung and liver tissues were evaluated for chemical:DNA adduct formation using 32 P-postlabeling. 1-Hydroxypyrene levels were 3.5 times greater in urine of DBA/2J than C57BL/6J mice, while a difference of only 1.3-fold was observed for urinary 3-hydroxybenzo[ a ]pyrene. Lung chemical:DNA adduct levels were greater in C57BL/6J than DBA/2J mice, with averages of 0.33 - 0.47 and 0.16 - 0.12 pmole adducts/mg DNA, respectively. Chemical:DNA adduct levels for liver tissues, however, were 2.5 times greater for DBA/2J than C57BL/6J mice. These results are contrary to those obtained in previous studies where a single hydrocarbon was administered. The abundance of PAHs present in coal tar may have evoked metabolic responses not governed by the Ah receptor, thereby accounting for the contradictory results.
Databáze:	OpenAIRE
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