Isolation of Intact Plastids from a Range of Plant Tissues

Autor:	Benjamin J. Miflin, Harry Beevers
Rok vydání:	1974
Předmět:	Spinacia Triose-phosphate isomerase activity biology Physiology fungi Ricinus food and beverages Plant Science biology.organism_classification Pisum Endosperm Biochemistry Botany Genetics Spinach Centrifugation Plastid
Zdroj:	Plant Physiology. 53:870-874
ISSN:	1532-2548 0032-0889
DOI:	10.1104/pp.53.6.870
Popis:	A technique for the isolation of intact plastids from spinach (Spinacia oleracea) and pea (Pisum sativum) leaves, pea roots and castor bean (Ricinus communis) endosperm is described. This technique involves brief centrifugation of whole homogenates on density gradients. Intact plastids were located in the gradient by assaying for triose phosphate isomerase activity. Contamination of the plastic peak with mitochondria and microbodies was estimated by measurement of cytochrome oxidase and catalase, respectively. For three of the four tissues the level of contamination of the plastids by these organelles was 2% or less. The sedimentation behavior of microbodies from different tissues is discussed.
Databáze:	OpenAIRE
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