Regucalcin is widely distributed in the male reproductive tract and exerts a suppressive effect on in vitro sperm capacitation in the water buffalo (Bubalus bubalis)
| Autor: | Arumugam Kumaresan, Mehtab S. Parmar, Harikrishna Pillai, S.K. Bhure, Taru Sharma G, A. M. Shende, Anjaneya A, L. Sreela |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty 030219 obstetrics & reproductive medicine Semen Cell Biology Biology Epididymis Regucalcin Sperm 03 medical and health sciences Bulbourethral gland 030104 developmental biology 0302 clinical medicine Seminal vesicle medicine.anatomical_structure Endocrinology Capacitation Internal medicine Genetics medicine Spermatogenesis Developmental Biology |
| Zdroj: | Molecular Reproduction and Development. 84:212-221 |
| ISSN: | 1040-452X |
| DOI: | 10.1002/mrd.22767 |
| Popis: | Regucalcin is a multi-functional, calcium-binding protein with roles in calcium homeostasis, apoptosis, cell proliferation, and free radical neutralization. Regucalcin is broadly expressed in the male reproductive organs of rat and bovine; here, we report its expression in the reproductive tract of male buffalo-especially in testis, epididymis, seminal vesicle, prostate, and bulbourethral gland of buffalo-as analyzed by real-time PCR, Western blot, and immunolocalization. Regucalcin degradation in seminal plasma, despite its high abundance in vesicular fluid, was demonstrated using recombinant regucalcin co-incubated with buffalo seminal plasma. This depletion of regucalcin appears to be related to its suppressive effect on in vitro sperm capacitation, observed using the chlortetracycline assay after treating buffalo spermatozoa with recombinant protein. Indeed, addition of recombinant regucalcin to capacitating media significantly reduced (P |
| Databáze: | OpenAIRE |
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