| Autor: |
Jang-Ho Hahn, Woon-Chul Shin, Hyun-Soon Kim, Mun-Sik Shin, Hyeon-Jung Kang, So-Hyeon Baek, Gang-Seob Lee, Chung-Kon Kim, Chun-Sun Seo |
| Rok vydání: |
2006 |
| Předmět: |
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| Zdroj: |
Journal of Plant Biotechnology. 33:309-313 |
| ISSN: |
1229-2818 |
| Popis: |
Discovery of single nucleotide polymorphisms (SNPs), including small insertions and deletions, is one of the hot topics in genetic research. The most common type of sequence variant consists of single base differences or small insertions and deletions at specific nucleotide positions. Significance of SNPs in rice is increasing for genetic research, positional cloning and molecular breeding. 170 lines and 194 lines derived from Sangjuchalbyeo/HR13721-53-3-1-3-3-2-2 Were used for Searching SNP markers related to bacterial blight resistance. Sangjuchalbyeo is susceptible to bacterial blight, but HR13721-53-3-1-3-3-2-2 has Xa1 gene resistant to bacterial blight. Individual lines were inoculated with race of bacterial blight and resistant or susceptible was evaluated after 3 weeks from inoculation. The genotypes of population were analysed by PCR-RFLP for SNP marker developing. The segregation of population showed almost 3:1, 1:1 ratio, respectively. Analysis of genotype using SNP marker is capable of confirming resistance for race and genotype through amplifying the gene using 16PFXal primer and digested the PCR product with Eco RV. There were close relation between resistance test for race and SNP marker genotype. Especially, DNA analysis using SNP marker is capable of judging homozygote/heterozygote in population compared with resistant test for Kl race. So, it seems to improve the selection efficiency in disease resistant breeding. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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