Array-Based N-Glycan Profiling of Cells in Culture
| Autor: | Anand Mehta, Richard R. Drake, Peggi M. Angel |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
PNGase F
0303 health sciences Cell type Chemistry 010401 analytical chemistry Peptide N-Glycosidase F 01 natural sciences Mass spectrometry imaging 0104 chemical sciences Cell biology carbohydrates (lipids) Glycomics 03 medical and health sciences Tissue sections Glycan profiling Cell culture 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071612408 |
| DOI: | 10.1007/978-1-0716-1241-5_23 |
| Popis: | N-glycan imaging mass spectrometry (N-glycan IMS) enables the detection and characterization of N-glycans in thin histological tissue sections. N-glycan IMS is used to study N-glycan regulation and localization in tissue-specific regions, such as tumor and normal adjacent to tumor, or by cell type within a tissue. Once a specific tissue-localized N-glycan signature is found to be associated with by a disease state, it has been challenging to study modulation of the same N-glycan signature by conventional molecular biology techniques. Here we describe a protocol that adapts tissue N-glycan IMS analysis workflows to cells grown on glass slides in an array format. Cells are grown under normal conditions in a cell culture chamber, fixed to maintain normal morphology, and sprayed with a thin coating of PNGase F to release N-glycans for imaging mass spectrometry profiling. |
| Databáze: | OpenAIRE |
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