Cytotoxic T lymphocyte-associated antigen-4 inhibits integrin-mediated stimulation

Autor:	Vanessa Ubaldi, Fiorella Di Nicuolo, Gino Doria, Lucia Gatta, Luigia Pace, Claudio Pioli, Gabriella Calviello
Rok vydání:	2002
Předmět:	biology Lymphocyte CD3 Immunology T-cell receptor CD28 hemic and immune systems chemical and pharmacologic phenomena Cell biology medicine.anatomical_structure Antigen medicine biology.protein Immunology and Allergy Cytotoxic T cell Cell activation Receptor
Zdroj:	Immunology. 107:209-216
ISSN:	1365-2567 0019-2805
Popis:	The negative role exerted by cytotoxic T lymphocyte-associated antigen-4 (CTLA-4) in the regulation of T-cell activity, as induced by T-cell receptor (TCR)/CD3 and CD28 costimulation, has been widely described. In the present work we investigated the role of CTLA-4 in the control of cell activation, as induced by costimulation of the adhesion molecule lymphocyte function-associated antigen-1 (LFA-1) in murine CD4+ T cells. Results show that CTLA-4 engagement inhibits interleukin-2 (IL-2) production, not only when induced by CD3/CD28 costimulation, but also when CD4+ T cells are costimulated by anti-CD3 and anti-LFA-1 monoclonal antibodies (mAbs). LFA-1 has been described to induce Ca2+ mobilization also in the absence of TCR engagement. Moreover, we found that CTLA-4 engagement negatively affects Ca2+ mobilization and NF-AT activation, as induced by LFA-1 engagement alone. PLCgamma1 phosphorylation was also dampened within minutes after CTLA-4 engagement. Altogether these data indicate that through the control of signals induced by different receptors, CTLA-4 could be a global attenuator of T-cell activation.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::322666fe99a5a486bb99e35ffd483c61 https://doi.org/10.1046/j.1365-2567.2002.01493.x Zobrazit plný text záznamu Plný text ve formátu PDF