Proline as a Formic Acid Stress Protectant During Fermentation of Glucose to Ethanol bySaccharomycesspp
| Autor: | Chenyu Du, C. E. Oshoma, Chris D. Powell, Katherine A. Smart, Trevor G. Phister |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ethanol biology Formic acid Saccharomyces cerevisiae food and beverages biology.organism_classification Saccharomyces Yeast 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Biochemistry Ethanol fuel Fermentation Proline Biotechnology |
| Zdroj: | Industrial Biotechnology. 13:209-216 |
| ISSN: | 1931-8421 1550-9087 |
| Popis: | During bioethanol production from lignocellulosic hydrolysates, yeasts are frequently exposed to various forms of fermentation stress. These include nutritional starvation, metabolites production, and fermentation-inhibiting compounds produced during pretreatment and hydrolysis of the lignocellulosic material. These inhibitors hamper efficient ethanol production as they can be toxic to the microbes conducting the fermentation. Formic acid is a significant inhibitor released into the hydrolyzates at a concentration of 10–30 mM. Previously it was found that proline acts as a compatible solute that enhances yeast tolerance to stress and therefore improves fermentation efficiency. Proline functions in vitro in protein and membrane stabilization, lowering Tm of DNA and scavenging of reactive oxygen species. In this study, the addition of proline to the media in order to improve the tolerance of yeast to formic acid during fermentation was investigated using Saccharomyces cerevisiae NCYC2592 and Saccha... |
| Databáze: | OpenAIRE |
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