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Summary An efficient micropropagation protocol was developed for Ricinus communis , which is a recalcitrant plant species. For this purpose, apical and axillary bud explants of two months old plantlets were cultured on modified MS medium. Shoot regeneration was optimal with 1000 nM BAP and 30 g · L -1 sucrose in the medium. Addition of NAA affected the number of shoots per explant, as well as shoot elongation, but no improvement of overall shoot proliferation was obtained. Elongated shoots were efficiently rooted after transfer to medium containing 100 nM IBA. Higher IBA concentration inhibited the number of roots formed, as well as the elongation of roots. The optimised migropropagation protocol for Ricinus , which is the first reported for this recalcitrant plant, now offers the possibility to use cell culture techniques for vegetative propagation and opens the door to introduce new genes via agro-bacterium-mediated transformation. |
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