Toxicity studies of agarwood essential oil in vero cells using electrical impedance sensor
| Autor: | Taufik Hakim Hamdan, Phirdaous Abbas, Yumi Zuhanis Has-Yun Hashim, Ahmad Fairuzabadi Mohd Mansor, Anis Nurashikin Nordin |
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| Rok vydání: | 2017 |
| Předmět: |
General Mathematics
General Physics and Astronomy Positive control macromolecular substances 02 engineering and technology engineering.material 01 natural sciences General Biochemistry Genetics and Molecular Biology law.invention chemistry.chemical_compound law Essential oil Chromatography 010401 analytical chemistry technology industry and agriculture General Chemistry Agarwood 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Cell culture Toxicity engineering Vero cell 0210 nano-technology General Agricultural and Biological Sciences Biosensor Toxicant |
| Zdroj: | Malaysian Journal of Fundamental and Applied Sciences. 13:540-545 |
| ISSN: | 2289-599X 2289-5981 |
| Popis: | Natural products have traditionally been used for medicinal purposes in Asian communities. Toxicity studies typically use animal testing to predict the harmfulness of a particular substance to human health. For this study, in lieu of animal testing, we utilize cell-based biosensors to evaluate the toxicity of natural products. The cell-based biosensors are fabricated on a printed circuit board with copper electrodes and are equipped with PDMS cell culture chambers. Two different electrodes (interdigitated and circular) were designed. Vero cells were used to represent normal healthy cells. The cells are first cultured on biosensors and then are inoculated with natural products, Taxol (chemo drug – positive control) and DMSO (negative control). Impedances of these biosensors were then recorded at six-hour intervals for 80 hours to determine the growth of the cells. It was found that compared to Taxol, natural products has a very low toxicant values. |
| Databáze: | OpenAIRE |
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