AZOXYSTROBIN INDUCES CHROMOSOMAL ABERRATIONS IN ROOTS OF THE HYDROPHYTE Bidens laevis L
| Autor: | Germán Lukaszewicz, Jorge Alberto Tognetti, Débora Jesabel Pérez, Mirta Lujan Menone |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Chlorophyll b
biology Bidens laevis food and beverages 010501 environmental sciences Pesticide biology.organism_classification Photosynthesis 01 natural sciences Pollution Horticulture chemistry.chemical_compound chemistry Azoxystrobin Aquatic plant Chlorophyll Waste Management and Disposal Chlorophyll fluorescence 0105 earth and related environmental sciences |
| Zdroj: | Revista Internacional de Contaminación Ambiental. 35:553-563 |
| ISSN: | 0188-4999 |
| DOI: | 10.20937/rica.2019.35.03.03 |
| Popis: | Aquatic plants can be used as in situ bioindicators of water quality due to their ability to accumulate agrochemicals. However, these plants may be at significant ecotoxicological risk from pesticides applied in crop fields. The fungicide azoxystrobin (AZX) induces DNA damage and changes in the antioxidant enzymatic system in leaves of the submerged hydrophyte Myriophyllum quitense . In the present study, the objectives were to determine whether AZX induces chromosomal damage in roots of the wetland plant Bidens laevis and to evaluate whether the damage in the antioxidant system was associated with damage in the photosynthetic system. Two experiments were carried out to evaluate the short-term effects (1 or 2 days) of the exposure to different concentrations of the active ingredient of AZX (0, 0.1, 1, 10, 50, 100 µg/L) on intact B. laevis plants. In the first experiment, photosynthetic biomarkers, including chlorophyll a fluorescence, SPAD units, and chlorophyll a and b content, were measured. In the second experiment, the frequency of chromosome aberrations (CA) was evaluated in root tips. In general, the photosynthetic biomarkers evaluated were not affected by AZX, except for a decrease in chlorophyll b content at 100 µg/L. Total CA increased at all AZX concentrations and the most frequent abnormalities were laggards and chromosomes not congregated at the metaphase equator, both indicators of spindle disturbance. This is the first report of AZX-induced chromosomal aberrations in a wetland plant exposed to environmentally relevant concentrations of the fungicide. The reason why AZX exposure had only a limited impact on biomarkers of the photosynthetic apparatus is discussed. |
| Databáze: | OpenAIRE |
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