INVESTIGATION OF MIGRATION BEHAVIORS OF PEPTIDES AND PROTEINS BY CAPILLARY ZONE ELECTROPHORESIS

Autor:	Masaaki Senda, Yasuyuki Kurosu, Yasuyo Sato
Rok vydání:	1991
Předmět:	chemistry.chemical_classification Capillary electrophoresis Isoelectric point chemistry Isoelectric focusing Capillary action Analytical chemistry Molecule Peptide Radius Small molecule Analytical Chemistry
Zdroj:	Analytical Sciences. 7:273-276
ISSN:	1348-2246 0910-6340
Popis:	In this study, using a single solvent system (30mM sodium phosphate buffer at four different pH values: 2.0, 5.0, 7.0 and 10.0). we have investigated the migration behaviors for not only small molecules (more than 35 peptides varying in size from 2 to 42 amino adid residues and varying in charge from -1.44 to 6.93) but also large ones (more than 20 proteins varying in molecular weight from 5kDa to 480kDa and varying in isoelectric point from 4, 1 to 110) Though the size (radius) of a molecule has been difficult to evaluate, it showed best linear correlation at pH 2, 0 (r 0, 981) with respect to Mw2/3 as radius of a peptide. Furthermore it was observed that a good correlation between observed and calculated mobility was only up to approximately 20kDa, using Mw2/3 for R and Z (calculated from the pKa of amino acid residues) at pH 2.0.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::31d6ada7c1a926f23f864236f32f0b13 https://doi.org/10.2116/analsci.7.supple_273 Zobrazit plný text záznamu Full text from SpringerLink