A near-infrared ratiometric fluorescent probe with large stokes based on isophorone for rapid detection of ClO− and its bioimaging in cell and mice
| Autor: | Yongbin Zhang, Yongfei Huang, Jianbin Chao, Fangjun Huo, Caixia Yin |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Physiological function Hypochlorous acid Near-infrared spectroscopy Metals and Alloys food and beverages 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology Condensed Matter Physics 01 natural sciences Rapid detection Fluorescence 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials chemistry.chemical_compound chemistry Clinical diagnosis Materials Chemistry Biophysics Electrical and Electronic Engineering 0210 nano-technology Instrumentation Isophorone |
| Zdroj: | Sensors and Actuators B: Chemical. 287:453-458 |
| ISSN: | 0925-4005 |
| DOI: | 10.1016/j.snb.2019.02.075 |
| Popis: | The reactive oxygen species (ROS) including hypochlorous acid (HOCl), on the one hand they can regulate physiological function in our living organisms, on the other hand abnormal concentration of ROS would induce a series of biomacromolecules damage and further endanger virtually all aspects of human health. Especially it can be directly related to cancer. Thus to elucidate and visualize their concentration is very imminent. Before many fluorescent probes were used to detect HOCl. In recent years, near-infrared or ratiometric fluorescent developed prosperously. The strategy of combining near-infrared and ratiometric fluorescent probe is overwhelming in the design of fluorescent probes. In this work, we successfully designed and obtained a near-infrared and ratiometric fluorescent probe with large stokes by reacting isophorone with coumarin, which can monitor HOCl high efficiently with quick response and low detection limit. In addition, cell imaging experiments show that the probe can identify endogenous and exogenous ClO− successfully, and nude mouse imaging experiments show that the probe can detect exogenous ClO−. It is possible that the probe can be applied in early clinical diagnosis. |
| Databáze: | OpenAIRE |
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