| Autor: |
G.D. Hunter, R.A. Gibbons, R.H. Kimberlin, G.C. Millson |
| Rok vydání: |
1971 |
| Předmět: |
|
| Zdroj: |
Journal of Comparative Pathology. 81:23-32 |
| ISSN: |
0021-9975 |
| DOI: |
10.1016/0021-9975(71)90051-x |
| Popis: |
A detailed study has been made of the effect of various solvents and detergents on preparations containing the scrapie agent, mainly membrane mixtures obtained by water washing a combined mitochondrial-microsome fraction. Reagents such as 2-chloroethanol and sodium dodecyl sulphate produce the most marked reductions in scrapie titre. These reagents are also the most potent in effecting the dissociation of complex membrane structures. Electrophoresis carried out on columns of Bio-Gel P-300 has shown that scrapie activity is associated with anodic membrane fragments. However, this activity does not appear to be associated exclusively with any one group of complex polysaccharide molecules. The overall conclusions drawn from these studies are consistent with the predictions of the membrane hypothesis of scrapie. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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