Cytokine production in response to endodontic infection in germ-free mice

Autor: M. de Uzeda, Jacques Robert Nicoli, A. P. Ribeiro Sobrinho, L.M. Farias, Leda Quercia Vieira, S. M. De Melo Maltos, M.A.R. De Carvalho
Rok vydání: 2002
Předmět:
Zdroj: Oral Microbiology and Immunology. 17:344-353
ISSN: 0902-0055
DOI: 10.1034/j.1399-302x.2002.170603.x
Popis: This study evaluated the cytokine profiles (type 1 or type 2) that are triggered by and modulate endodontic periapical infections in the root canal system of germ-free mice. Microorganisms isolated from two patients with pulpal necrosis were inoculated into two groups of experimental animals: group I (Gemella morbillorum) and group II (Bifidobacterium adolescentis, Fusobacterium nucleatum and Clostridium butyricum). In vitro, G. morbillorum induced type 1 cytokine synthesis, while the modulation processed in vivo seemed to have the opposite effect, with a reduction in the basal levels of IL-12 and IFN-gamma, IL-4-independent down-modulation. In vitro, microorganisms from group II, in poly-infection, induced a reduction of type 1 cytokine levels from day 10 to day 20, which seemed to be modulated via IL-4. In vivo, however, a predominance of the immune response to one species over the others occurred.
Databáze: OpenAIRE