Autor: | Tairo Hagishita, Masanobu Nishikawa, Tadashi Hatanaka |
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Rok vydání: | 2000 |
Předmět: |
chemistry.chemical_classification
Chromatography biology Chemistry Phospholipase D Phospholipid Substrate (chemistry) Bioengineering General Medicine Phospholipase biology.organism_classification Applied Microbiology and Biotechnology Hydrolysis chemistry.chemical_compound Enzyme Biochemistry Specific activity Bacteria Biotechnology |
Zdroj: | Biotechnology Letters. 22:1587-1590 |
ISSN: | 0141-5492 |
DOI: | 10.1023/a:1005644032415 |
Popis: | The transphosphatidylation and hydrolytic activities of phospholipase d in culture supernatants of soil isolates were evaluated by a specific spectrophotometric method for quantitative determination using an artifical substrate, phosphatidyl-p-nitrophenol. Phospholipase d from strain TH-2 showed the highest specific activity and ratio of transphosphatidylation activity to hydrolytic activity among those from the eight soil isolates and commercial Actinomycetes phospholipase d. |
Databáze: | OpenAIRE |
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