VARIATIONS IN THE QUANTITY OF TYPE II COLLAGEN IN CARPAL TUNNEL SYNDROME AND TRIGGER FINGER

Autor:	Arnold-Peter C. Weiss, Edward Akelman, Hans-J. Barrach, Adriel A. Kramer
Rok vydání:	1996
Předmět:	Pathology medicine.medical_specialty business.industry medicine.drug_class Cartilage Type II collagen General Medicine medicine.disease Monoclonal antibody Blot Tendon sheath medicine.anatomical_structure Metaplasia medicine Trigger finger medicine.symptom business Carpal tunnel syndrome
Zdroj:	Hand Surgery. :95-101
ISSN:	1793-6535 0218-8104
DOI:	10.1142/s0218810496000178
Popis:	Carpal tunnel syndrome (CTS) and trigger finger (TF) are two related disorders which involve alterations in the normal mechanical loading of tendon sheath pulleys. This study examines the presence of Type II collagen in the carpal ligament and A-1 pulley by Western blotting to determine the extent of cartilage metaplasia that may occur in these tissues. Cyanogen bromide peptides generated from tissue supplied from 77 patients were separated by SDS-PAGE and transferred to nitrocellulose. The membranes were stained with the E1E5 monoclonal antibody and collagen levels were quantified. All specimens were found to contain at least small amounts of Type II collagen. Approximately 15 percent of the samples from patients with carpal tunnel syndrome and trigger finger were found to contain significantly elevated levels of Type II collagen. All specimens with high levels of Type II collagen were found in female patients. These increases may indicate the presence of cartilage metaplasia enabling the tissue to counteract compressive loads. With further study, tissue staining techniques using monoclonal antibodies may provide useful clinical information on severity or long-term outcome prognosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::31bafdc90b9fd0db9b01ba22153383d9 https://doi.org/10.1142/s0218810496000178 Zobrazit plný text záznamu