Preparation of Plasma for Coagulation Testing: Evaluation of the StatSpin® High-Speed Centrifuge
| Autor: | J. E. Leveson, Scott R. Mayorga, Ellinor I. B. Peerschke, Linda Goldberg, A. J. Marengo-Rowe, Sherry Robinson, Ellen Hope |
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| Rok vydání: | 1991 |
| Předmět: | |
| Zdroj: | Laboratory Medicine. 22:190-193 |
| ISSN: | 1943-7730 0007-5027 |
| DOI: | 10.1093/labmed/22.3.190 |
| Popis: | Studies were performed to evaluate the StatSpin® high-speed centrifuge for processing whole blood for coagulation testing. Blood was obtained by venipuncture and collected into siliconized Vacutainer tubes containing sodium citrate (9 volumes of blood: 1 volume of 3.8% sodium citrate). Specimens were divided into two aliquots and centrifuged for 2 minutes (11,200 g, 22°C) in a StatSpin centrifuge (high speed) and for 20 minutes (1,000 g, 40°C) in a Beckman TJ6 centrifuge (low speed), or for 10 minutes (1,150 g, 22°C) in a Sorvall GLC-2 centrifuge (moderate speed). Excellent correlations were observed for prothrombin time (r=.992, n=152), activated partial thromboplastin time (r=0.971, n=146), and fibrinogen determinations (r=.947, n=38) performed with plasma derived by high- and lowor moderate-speed centrifugation. Plasma prepared by high-speed or moderate-speed centrifugation also gave similar thrombin time results (n=22). Examination of residual platelet counts of plasma obtained by highand low- or moderate-speed centrifugation revealed consistently lower residual platelet counts following StatSpin centrifugation. These data suggest that the StatSpin high-speed centrifuge can be used reliably to process wholeblood specimens for screening coagulation tests. Since the 2-minute centrifugation time of the StatSpin is considerably shorter than that of traditional lower speed centrifuges, the instrument is ideally suited for the processing of blood specimens for stat coagulation testing. |
| Databáze: | OpenAIRE |
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