Effect of cyclical stretching on cultured rat cerebral vascular smooth muscle cells: A flow cytometric, confocal laser scanning, and TEM Study
| Autor: | Thomas Waldschmidt, Elizabeth Yoder, Gary L. Baumbach, Shams Ghoneim, Steven A. Moore |
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| Rok vydání: | 1993 |
| Předmět: | |
| Zdroj: | Proceedings, annual meeting, Electron Microscopy Society of America. 51:426-427 |
| ISSN: | 2690-1315 0424-8201 |
| DOI: | 10.1017/s042482010014796x |
| Popis: | Vascular hypertrophy during chronic hypertension has been linked to a variety of potential determinants including increased intravascular pressure, neurohumoral factors and genetic factors. Of the various determinants, one might assume that arterial pressure per se would play an especially important role. Nonetheless, the role of intravascular pressure has remained unclear. Based on findings in vivo, we recently proposed that increases in pulse pressure, as opposed to mean pressure, may be an important determinant of cerebral vascular hypertrophy (3).The goal of the present study was to further explore the possible link between pulse pressure and hypertrophy using a combination of tissue culture, fluorescent-activated cell sorting (FACS), transmission electron microscopy, and confocal laser fluorescent microscopy techniques. Cultured smooth muscle cells derived from rat cerebral microvessels were used for these experiments. Flex culture plates with rigid (control) or flexible (elastomer), collagen-coated bottoms were seeded with smooth muscle cells. After 48 hours to allow for attachment, the culture plates were placed in a Flexercell Strain Unit (model FX-2000) (1, 2), and exposed to continuous cyclical stretching at 20 k Pascals unit pressure and 60 cycles per minute for 5 days. |
| Databáze: | OpenAIRE |
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