| Autor: |
Sang-Gyu Park, Byung Keuk Kim, Seung-In Lee, Hak Soo Suh, Youl-Lae Jo, Suk-Chul Suh |
| Rok vydání: |
2002 |
| Předmět: |
|
| Zdroj: |
Korea Journal of Environmental Agriculture. 21:156-161 |
| ISSN: |
1225-3537 |
| DOI: |
10.5338/kjea.2002.21.2.156 |
| Popis: |
Two different cDNA clones for superoxide dismutase (SOD) were isolated from an weedy rice variety (Oryza sativa, cv. Bhutan14Ad) and were introduced into a cultivated rice variety (Oryza sativa, cv. Nakdong) in order to develop the environmental stress-resistant rice plants. Sequence analysis of the cloned cDNAS indicated that the deduced amino acid sequence of SOD-A is 88.4% identical to that of SOD-B. Furthermore, the nucleotide sequence of SOD-A is 99.3% identical to that of a Cu/Zn SOD gene of Oryza sativa (GenBank accession No. L36320). The nueleotide sequence of SOD-B was identical to that of the previously published SOD gene (Accession No. D01000). A cultivated rice variety, Nakdong-byeo, was transformed with chimeric SOD genes containing a actin promoter of rice and pin2 terminator using a particle bombardment technique. Transformed calli were selected on an selection medium containing phosphinothricin (PPT). Transgenic rice plants were regenerated from the PPT-resistant calli. PCR analysis with genomic DNAs from transgenic plants revealed that transgenes are introduced into rice genome. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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