Screening of Compounds for Activity against HIV: A Collaborative Study
| Autor: | Abraham Karpas, D. J. Jeffries, T. J. O'Connor, Rudi Pauwels, Naheed Mahmood, E. De Clercq, Alan J. Hay, H. C. Holmes, Jan Desmyter, Juraj Petrik, D. Kinchington |
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| Rok vydání: | 1991 |
| Předmět: |
0301 basic medicine
Screening test business.industry Validation test 030106 microbiology Human immunodeficiency virus (HIV) General Medicine medicine.disease_cause 01 natural sciences Virology Virus 0104 chemical sciences 010404 medicinal & biomolecular chemistry 03 medical and health sciences Investigation methods Multicenter study Immunology Medicine business |
| Zdroj: | Antiviral Chemistry and Chemotherapy. 2:287-293 |
| ISSN: | 2040-2066 |
| DOI: | 10.1177/095632029100200503 |
| Popis: | The collaborative study was undertaken to examine the sensitivity of a range of tests used in assessing the antiviral activities of compounds against human immunodeficiency virus (HIV). A panel of 20 compounds with diverse antiviral activities against HIV were tested under code at three antiviral testing centres supported by the Medical Research Council's AIDS Directed Programme and at the European Community Centralised Facility (ECCF) for New Antiviral Compounds against AIDS in Belgium. Compounds known to have major anti-HIV activity ranked high in all assays, with the exception of the glucosidase inhibitors and certain nucleoside analogues. Results of two assays based on MT4 cells (centre IV) showed a high degree of similarity, despite the use of distinct HIV-1 (HTLV-IIIB) and HIV-2 (ROD) viruses. Considerable similarity was also observed between the assays based on HTLV-IIIRF in C8166 cells (centres I and II). Other assays performed at centre II and at centre III had enhanced sensitivity for glycosidase inhibitors. The differences in anti-HIV activity that were observed may be attributable to specific properties of the cell lines used and particular testing methodologies. The use of more than one type of assay is advisable in order not to miss compounds with low to moderate activity against HIV. |
| Databáze: | OpenAIRE |
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