Studies on the Role of Ribosomal Proteins L7 and L12 in the in Vitro Synthesis of β-Galactosidase

Autor:	Nathan Brot, Herbert Weissbach, Hsiang-Fu Kung, Carlos Spears, J. Eugene Fox
Rok vydání:	1973
Předmět:	Eukaryotic Large Ribosomal Subunit food and beverages Cell Biology Biology Ribosomal RNA medicine.disease_cause Biochemistry Ribosome Molecular biology Ribosomal protein 28S ribosomal RNA medicine Eukaryotic Small Ribosomal Subunit Eukaryotic Ribosome Molecular Biology Escherichia coli
Zdroj:	Journal of Biological Chemistry. 248:5012-5015
ISSN:	0021-9258
Popis:	The DNA-directed in vitro synthesis of β-galactosidase has been examined in a system dependent on Escherichia coli ribosomes, a salt wash of the ribosomes, and a 200,000 x g supernatant fraction. The ribosomes can be replaced by 30 S and 50 S ribosomal subunits. Extraction of the 50 S particles with ethanol-NH4 yields inactive particles which can be reactivated equally well by the addition of either ribosomal protein L7 or L12.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::31960edb4f0ce8c046b3ff77328af74c https://doi.org/10.1016/s0021-9258(19)43664-8 Zobrazit plný text záznamu