Contribution of perfusion to the 11 C‐acetate signal in brown adipose tissue assessed by DCE‐MRI and 68 Ga‐DOTA PET in a rat model
| Autor: | André C. Carpentier, Mélanie Archambault, Martin Lepage, Réjean Lebel, Christophe Noll, Luc Tremblay, Gabriel Richard, Brigitte Guérin, Samia Ait-Mohand, Denis P. Blondin |
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| Rok vydání: | 2020 |
| Předmět: |
medicine.diagnostic_test
business.industry Rat model Blood volume 3. Good health 030218 nuclear medicine & medical imaging Gadobutrol 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine.anatomical_structure chemistry Pharmacokinetics Positron emission tomography Brown adipose tissue medicine DOTA Radiology Nuclear Medicine and imaging Nuclear medicine business Perfusion 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Magnetic Resonance in Medicine. 85:1625-1642 |
| ISSN: | 1522-2594 0740-3194 |
| Popis: | PURPOSE Determine if dynamic contrast enhanced (DCE) -MRI and/or 68 gallium 1,4,7,10-tetraazacyclododecane N, N', N″, N‴-tretraacetic acid (68 Ga-DOTA) positron emission tomography (PET) can assess perfusion in rat brown adipose tissue (BAT). Evaluate changes in perfusion between cold-stimulated and heat-inhibited BAT. Determine if the 11 C-acetate pharmacokinetic model can be constrained with perfusion information to improve assessment of BAT oxidative metabolism. METHODS Rats were split into three groups. In group 1 (N = 6), DCE-MRI with gadobutrol was compared directly to 68 Ga-DOTA PET following exposure to 10 °C for 48 h. 11 C-Acetate PET was also performed to assess oxidation. In group 2 (N = 4), only 68 Ga-DOTA PET was acquired following exposure to 10 °C for 48 h. Finally, in group 3 (N = 10), perfusion was assessed with DCE-MRI in rats exposed to 10 °C or 30 °C for 48 h, and oxidation was measured with 11 C-acetate. Perfusion was quantified with a two-compartment pharmacokinetic model, while oxidation was assessed by a four-compartment model. RESULTS DCE-MRI and 68 Ga-DOTA PET provided similar perfusion measures, but a decrease in the perfusion signal was noted with longer imaging sessions. Exposure to 10 °C or 30 °C did not affect the perfusion measures, but the 11 C-acetate signal increased in BAT at 10 °C. Without prior information about blood volume, the 11 C-acetate compartment model overestimated blood volume and underestimated oxidation in 10 °C BAT. CONCLUSION Precise assessment of oxidation via 11 C-acetate PET requires prior information about blood volume which can be obtained by DCE-MRI or 68 Ga-DOTA PET. Since perfusion can change rapidly, simultaneous PET-MRI would be preferred. |
| Databáze: | OpenAIRE |
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